Description
The Real-time PCR System YR06248 – YR06249, featuring cutting-edge signal gathering and spectrum separation technology, along with a unique temperature control system, achieves an advanced level of detection sensitivity. It offers multi-color crosstalk correction, temperature uniformity, and accuracy. This instrument is available in either a 4-channel or 5-channel configuration, compatible with a wide spectrum of qPCR dye-based and probe-based chemistry, allowing easy execution of multiplex PCR applications. Supporting standard analysis methods like absolute quantification, genotype, HRM, MMCA, and gene expression analysis, it provides rapid and precise thermal cycling, an innovative optical design, excellent reproducibility, and a dynamic range of up to 10 logs, along with high-resolution melting curve analysis.
Market Price
The typical market price range for real-time PCR systems such as the YR06248 and YR06249 is between $23,500 and $23,800 USD, offering a competitive option for laboratories seeking advanced features without compromising their budget.
Frequently Asked Questions
What is the difference between the 4-channel and 5-channel models? The main difference lies in the number of excitation lights and supported targets, where the 5-channel model offers an additional channel.
Is the system compatible with all types of PCR plates? Yes, it supports 8×0.1mL PCR strips, 96×0.1mL individual tubes, and 0.1mL PCR plates.
Advantages and Disadvantages
Advantages: The system offers exceptional sensitivity and innovative design, improving the throughput and accuracy of PCR results. Its compatibility with various chemistries ensures flexibility in applications.
Disadvantages: The initial investment can be high for smaller labs, and the complexity of the system might require additional staff training.
Use of the Product in the Field
This real-time PCR system is essential in research and clinical laboratories performing quantitative PCR assays. It accurately quantifies nucleic acids in diverse applications, such as pathogen detection, genetic variation analysis, and gene expression profiling.
Recommendations
To maximize the efficiency of the Real-time PCR System YR06248 – YR06249, ensure regular calibration and maintenance as recommended by the manufacturer. Staff should undergo comprehensive training to fully utilize the advanced features and capabilities of the system.
Features
- Fast and Precise Thermal Cycling
- Innovative Optic Design
- Excellent Reproducibility
- Up to 10-log dynamic range
- Melting Curve Analysis
- High Resolution
Technical Specifications
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
